Effects of recombinant epidermal growth factor receptor antisense adenovirus combined with irradiation on breast cnacer cells
- VernacularTitle:表皮生长因子受体反义重组腺病毒联合放射线对乳腺癌细胞的作用
- Author:
Yunjun LI
;
Lin MA
;
Jianli SUI
;
Pingkun ZHOU
- Publication Type:Journal Article
- Keywords:
Epidermal growth factor receptor;
RNA,antisense;
Adenoviruses, recombinant;
Cell line,breast neoplasms
- From:
Chinese Journal of Radiation Oncology
1995;0(02):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the effects of a recombinant antisense adeno virus for epidermal growth factor receptor (EGFR) combined with irradiation on b reast cancer cells.Methods Human EGFR cDNA fragment was subcloned in the oppos ite orientation to the cytomegaloviral promoter and inserted into a E1/E3-delet e d type 5 adenoviral vector to obtain AdE5 construct which expresses EGFR antisen se RNA. Combined with ?-ray irradiation, its effects on clonogenicity and cell cycle phase distribution were studied in a human breast cancer line MDA-MB-231 . Results EGFR protein expression was dramatically inhibited in MDA-MB-231 cell s after AdE5 infection. The post-irradiation clonogenicity was reduced by AdE5 in a viral and irradiation dose-dependent manner. Further cytometric analysis show e d that AdE5 infection at a?MOI of 300?pfu/cell induced a cell cycle progre ssion from radio-resistant G 0+G 1 phases to radiosensitive G 2+M phases, resultin g in a synergistic effect after combination of these two treatments. Conclusions The t ransduction of EGFR antisense RNA by adenoviral vector is effective for antisens e strategy targeting EGFR, and increases the cell-killing effect of ionizing radiation on breast cancer cells.
