Changes in NO content and NOS activity in serum and testis in diabetic rat
- VernacularTitle:糖尿病鼠血清及睾丸中一氧化氮含量和一氧化氮合酶活性的变化
- Author:
Zhihong DENG
;
Xiaoyan LIN
;
Wei WANG
- Publication Type:Journal Article
- Keywords:
diabetes mellitus;
insulin-dependent;
testis;
nitric oxide;
nitric oxide synthase
- From:
Medical Journal of Chinese People's Liberation Army
1982;0(03):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To observe the changes in NO content and NOS activity of serum and the testis in Wistar rats with type I diabetes mellitus. Methods 63 rats were randomly divided into control group(C) and diabetes group(D). Diabetes was induced by intraperitoneal injection of Alloxan. Rats with diabetes were observed for 1 week(D 1), 2 weeks(D 2), 3 weeks(D 3), 4 weeks(D 4), 5 weeks(D 5), 6 weeks(D 6), 7 weeks(D 7) and 8 weeks(D 8), respectively, and they were sacrificed at the end of each observation period. Serum was obtained and testis homogenates were prepared, and the levels of NO content and NOS activity of them were determined respectively. Results Compared diabetes group with control group, NO contents and NOS activity of serum from diabetic rats were higher, then NOS activity began to decline to approach the control level. In the testis homogenates, NO content dropped slightly after injection of Alloxan, while NOS activity showed no significant change. Thereafter, both NO content and NOS activity of testis homogenates were increased. Conclusion There are changes in NO content and NOS activity of serum and testis of rats after diabetes are induced with Alloxan.
