A study of N-ras expression in K562 cell line
- VernacularTitle:N-ras基因在K562细胞中的表达研究
- Author:
Xiang YAN
;
Wenli MA
;
Yanbin SONG
- Publication Type:Journal Article
- Keywords:
genes, ras;
K562 cells;
reverse transcriptase polymerase chain reaction;
sequence analysis, DNA
- From:
Medical Journal of Chinese People's Liberation Army
1982;0(03):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To explore the effect of the mutant and expression level of N-ras on chronic myelogenous leukemia. Method We investigated the mutant by direct sequencing in a K562 human chronic myelogenous leukemia cell line, with determination of the expression level of N-ras mRNA in K562 by RT-PCR. Result No single point mutation was detected in K562 cell line, furthermore, the expression level of N-ras gene is abnormaly high in contrast to normal human. Conclusion Our results indicated that the expression level of N-ras gene was obviously high in K562 cell line, and the underlying mechanism was not only mutation, so that further investigation is called for.
