Construction of antisense survivin eukaryotic expression vector and its expression in glioma cells
- VernacularTitle:Survivin反义核酸真核表达载体的构建及在胶质瘤细胞中的表达
- Author:
Zhicheng WANG
;
Lina ZHOU
;
Deyu GUO
;
Haidon LI
- Publication Type:Journal Article
- Keywords:
survivin gene;
antisense nucleic acid;
cell transfection;
glioma
- From:Journal of Third Military Medical University
1983;0(04):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To construct antisense survivin eukaryotic expression vector pIRES2-EGFP-SVVas and detect its expression in human malignant glioma cell line SHG-44 cells. Methods The antisense survivin eukaryotic expression vector pIRES2-EGFP-SVVas was constructed and then transfected into SHG-44 cells with lipofectamine. The expression of survivin on the cells was measured by RT-PCR and Western blotting. Results The mRNA and protein expression of survivin decreased in the transfected cells. Conclusion pIRES2-EGFP-SVVas can decrease the expression of survivin in the cells transfected with the vector.
