The influences of fenofibrate on levels of tumor necrosis factor-alpha in serum and adipocytes secretion of hypercholesterolemia rabbits
- VernacularTitle:非诺贝特对高脂血症兔血清及脂肪细胞分泌肿瘤坏死因子-?的影响
- Author:
Shuiping ZHAO
;
Jie WU
- Publication Type:Journal Article
- Keywords:
fenofibrate;
adipocytes;
TNF-?;
hypercholesterolemi a
- From:
Chinese Pharmacological Bulletin
1987;0(02):-
- CountryChina
- Language:Chinese
-
Abstract:
Aim Background tumor necrosis factor-alpha (TNF-?) is produced by monocyte-macrophages and adipocytes. It may provide the link between inflammation and atherosclerosis. The aim of this study was to evaluate the effect of fenofibrate on serum TNF-? concentration and TNF-? secretion by adipocytes from hypercholesterolemia rabbits.Methods Ten male New Zealand white rabbits were fed with high-cholesterol diet for 8 weeks, and were randomly divided into two groups: ①high cholesterol group: maintained cholesterol diet for 4 wks; ② fenofibrate group: the same cholesterol diet supplemented with fenofibrate (30 mg?kg?d -1) for 4 weeks. And control group was fed with normal diet for 12 weeks. Subcutaneous adipose was collected for adipocytes culture. TNF-? concentrations in serum and adipocytes culture supernatant were measured by ELISA. Adipocytes were harvested for semi-quantitative reverse transcription-polymerase chain reaction (RT-PCR) studies. Results :Rabbits fed with high-cholesterol diet showed higher serum levels of total cholesterol, low density lipoprotein cholesterol than those fed with normal diet (P
