Screening of small molecules inducing PC12 cell differentiation
- VernacularTitle:促神经细胞分化小分子活性物质的筛选研究
- Author:
Hong YIN
;
Guanhua DU
- Publication Type:Journal Article
- Keywords:
inducing cell differentiation;
PC12 cells;
drug scree n;
small molecule
- From:
Chinese Pharmacological Bulletin
1987;0(02):-
- CountryChina
- Language:Chinese
-
Abstract:
Aim To search for small molecule NGF mimetic s using a primary screening model.Methods Both rapid quantitati ve bioassay for NGF(-like) activity by measurement of neurite extension in NGF -induced PC12 cells and a following MTT technique to monitor cells alive were u sed.Results More than 1 600 compounds were tested using the se methods and several effective samples with lower cytotoxicity were found. One of them, the compound B,was in good dose-and time-effect relationship. Conclusion All above studies provide a useful model and chemical info rmation for further screen of neurotrophic mimetics and neural cell tumor induce rs.
