The effects of aminophylline on mRNA expression of interleukin-5, interleukin -3 and granulocyte-macrophage colony-stimulating factor receptors in eosinop hils of asthmatic guinea pigs
- VernacularTitle:氨茶碱对哮喘豚鼠嗜酸细胞IL-3、IL-5及GM-CSF受体表达的影响
- Author:
Zhikui LI
;
Gang LIU
;
Changzheng WANG
;
Guisheng QIAN
- Publication Type:Journal Article
- Keywords:
amnophylline;
eosinophil;
interleukin;
receptor;
apoptosi s
- From:
Chinese Pharmacological Bulletin
1987;0(02):-
- CountryChina
- Language:Chinese
-
Abstract:
Aim To investigate the effect of Aminophyllin e(AM) on expression of Interleukin-5 receptor ?(IL-5R?), IL-3R?, granulocy te- macrophage-colony-stimulating factor receptor ?(GM-CSFR?) and common ? receptor(?cR) in eosinophils(Eos) of BALF in asthmatic guinea pigs, and mech anism of AM promoting Eos apoptosis. Methods 18 guinea pigs wer e divided into three groups randomly, normal control group, asthma group and AM -treated group. Asthma model of guinea pigs was sensitized by ovalbumin. Hypode nse Eos(HEos) and normodense Eos(NEos) were purified from BALF by gradients of p ercoll. Apoptosis was detected by TUNEL. mRNA expression of IL-5R?, IL-3R?, GM-CSFR? and ?cR in Eos were measured by RT-PCR and hybridization.Re sults Apoptosis of HEos and NEos in asthma group(4?2,3?2) were low er than that in normal group(8?2,7?2)(P
