Anti-oxidative and mitochondria-protective effects of extract of astragalus on focal cerebral ischemia-reperfusion injury in rats
- VernacularTitle:黄芪提取物对局灶性脑缺血再灌注损伤的抗氧化及线粒体保护作用
- Author:
Xiaofen MIN
;
Weiping LI
;
Shaobin WANG
;
Ting HE
;
Yanyan YIN
;
Liang MING
- Publication Type:Journal Article
- Keywords:
extract of astragalus;
cerebral ischemia/reperfusion;
free radical;
mitochondrion
- From:
Chinese Pharmacological Bulletin
1987;0(02):-
- CountryChina
- Language:Chinese
-
Abstract:
Aim To study the anti-oxidative and mitochondria-protective effects of estract of astragalus (EA ) on focal cerebral ischemia-reperfusion injury and its mechanism in rats.Methods Middle cerebral artery occlusion(MACO) was used to induce focal cerebral ischemia-reperfusion model. After 24 h reperfusion,MDA, LD content and SOD activity of brain homogenate were tested. MDA content,SOD and ATPase activity in mitochondrion were also tested.Transmission electron microscopy was used to assess the ultrastructure dstruction. Results EA(20, 40, 80 mg?kg -1) significantly inhibited the increase of MDA, LD after cerebral ischemia-reperfusion. EA (40, 80 mg?kg -1) also inhibited the decrease of activit of SOD in rats. EA(20, 40, 80 mg?kg -1) inhibited the increase of MDA and inhibited the decrease of activities of SOD, Na+,K+-ATPase, Ca 2+,Mg 2+-ATPase in mitochondrion. The examination by transmission electron microscopy showed that EA (20, 40 mg?kg -1) protected the ultrastructure destruction. Conclusion EA had protective effects against focal cerebral ischemia reperfusion injuries via attenuating cerebral oxygen free radical(OFR)lipid peroxidation and protecting mitochondria.
