Calcium activator induced the expression of myocardium hypertrophy transcription factor
- VernacularTitle:钙激动剂诱导心肌肥大转录因子的表达
- Author:
Yongjian YANG
- Publication Type:Journal Article
- Keywords:
myocardial diseases;
transcription factors;
calcium activators
- From:
Chinese Pharmacological Bulletin
1987;0(02):-
- CountryChina
- Language:Chinese
-
Abstract:
Aim To investigate the role of [Ca 2+] i form different origins in the course of myocardial hypertrophy mediated by calcineurin (CaN)- nuclear activated T cell factor(NFAT 3) signal transduction.Methods The primarily cultured crdio myocyte were irritated with angiotensin(Ang) Ⅱ and ryanodine(RY) which Cause Ca 2+ inflow and release respectively. Then to observe the changes of (CaN)-NFAT 3 path way were then observed. Western blotting was employed to se mi-quantify CaN, NFAT 3 and zinic finger transcription factor(GATA 4). 3H-Leu incorporation was used as an index of myocyte hypertrophy. Cyclosporin A (CsA) was applied to restrain CaN-NFAT 3 pathway as a kind of CaN-selective antagonist.Results CaN, NFA T 3, GATA 4 expression significantly increased 1 and 3 days after the stimulation of cardiomyocytes with Ang Ⅱ and RY(10 -7 mol?L -1) as compared with that of a control group (P
