Effects of procyanidins on apoptosis of PC12 cells induced by A?_(25-35) and its mechanism
- VernacularTitle:原花青素对A?_(25-35)诱导PC12细胞凋亡的影响及其机制的研究
- Author:
Hanfang MEI
;
Zhaoyang XIE
;
Qifeng ZHU
- Publication Type:Journal Article
- Keywords:
?amyloid peptide;
procyanidins;
PC12 cells;
apoptosis;
P53;
bcl-2
- From:
Chinese Pharmacological Bulletin
1987;0(02):-
- CountryChina
- Language:Chinese
-
Abstract:
Aim To study the effects of Procyanidins (PC) on apoptosis of PC12 cells induced by A? 25-35. Methods Cell survival rate was evaluated by MTT assay, and apoptosis was analyzed by Hoechst-PI fluorescence staining. The expressions of mRNA and protein for P53 and Bcl-2 were tested by RT-PCR and Western blot. Results Pretreatment with different concentrations of PC (10~30 mg?L -1) for 1h increased the survival rate of PC12 cells in a dose-dependent fashion. PC prevented the PC12 cells nuclei from shrinkage, condensation and cleavage induced by A? 25-35. PC decreased the expressions of P53 mRNA and P53 protein, and increased the expression of bcl-2 mRNA and Bcl-2 protein. Conclusion These results indicated that PC can protect PC12 cells from apoptosis induced by A? 25-35. The mechanism of protection is likely related to decreasing P53 gene expression, and increasing bcl-2 gene expression.
