EFFECTS OF DIETARY SUPPLEMENTATION OF ANTIOXIDANTS ON zf-9 mRNA EXPRESSION IN RAT HEPATIC STELLATE CELLS DURING ACUTE LIVER DAMAGE
- VernacularTitle:补充抗氧化剂对急性肝损伤大鼠肝星型细胞zf-9mRNA表达的影响
- Author:
Liangmin XIE
;
Lin MA
;
Wufeng CHEN
;
Xiuhua SHEN
;
Jianqin SUN
- Publication Type:Journal Article
- Keywords:
antioxidant;
hepatic stellate cell;
zf-9;
VE;
Se;
quercetin
- From:
Acta Nutrimenta Sinica
1956;0(01):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To investigate the effect of vitamin E, selenium and quercetin on the expression of zf-9 mRNA of hepatic stellate cells (HSC) during early acute liver injury in rats. Methods:90 healthy SD rats were randomly divided into four groups: normal control, pathological control, VE 20 mg/(kg bw﹒d) + Se 16 ?g/(kgbw﹒d) supplement, and quercetin 50 mg/(kg bw﹒d) supplement through intragastric way for 15 d respectively. The normal control group was injected with normal saline, the others were given intraperitonal CCl4 once to induce acute liver injury model. The serum levels of MDA, SOD, ALT and AST were determined at 3 h, 6 h, 12 h and 24 h after CCl4 injection respectively. The pronase-collagenase perfusion of liver was used to isolate HSC, and the expression of zf-9 mRNA was examined by RT-PCR. Results: Serum levels of MDA were lower in VE+Se supplement group and quercetin supplement group than pathological group. Supplementation with VE+Se and quercetin could decrease the level of ALT and AST and down-regulate the expression of zf-9 mRNA. Conclusion:Dietary supplementation of VE,Se and quercetin could effectively down-regulate the expression of zf-9 mRNA in HSC during early acute liver injury in rats.
