EFFECTS OF GRAPE PROCYANIDINS ON FREE RADICAL INDUCED ENDOTHELIAL CELL DAMAGE
- VernacularTitle:葡多酚对血管内皮细胞自由基损伤的影响
- Author:
Na NA
;
Peilong XU
;
Jinyi ZHONG
- Publication Type:Journal Article
- Keywords:
grape procyanidins (GPC);
oxygen free radicals;
endothelial cell
- From:
Acta Nutrimenta Sinica
1956;0(01):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective:The protective effects of grape procyanidins (GPC) on free radical induced endothelial cell damage were investigated. Methods:Human umbilical vein vascular endothelial cells (ECV304) were cultured in GPC of different doses. Oxidative injury was caused by Fenton system, and MTT method was used to test cell proliferation when cells were cultured 12, 24, 36, 48 h, respectively, MDA and SOD in cells were also determinated 30 min after Fenton system was added. Results: GPC protected cell proliferation damage induced by OH. When GPC doses increased, the protective effects were . enhanced, while MDA level and apoptosis rate of 100?g/ml GPC group both decreased (P
