Gene transfection and expression of murine liver-specific microRNA in HeLa cell line
- VernacularTitle:小鼠肝脏特异的微RNA在HeLa细胞中的转染与表达
- Author:
Jianguo SUN
;
Rongxia LIAO
;
Zhengtang CHEN
;
Jianxi ZHOU
- Publication Type:Journal Article
- Keywords:
microRNA;
small interfering RNA;
non-coding RNA;
eukaryotic expression
- From:Journal of Third Military Medical University
1983;0(03):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To express miR-122 in HeLa cells. Methods pAVU6+27-mir122, an eukaryotic expression vector of miR-122, was transfected into HeLa cell line by electroporation. G418-resistant clones were screened for cell clones with stable transfection. Northern blotting and dot blotting were used to detect miR-122 expression in the transfected HeLa cells. Results Northern blot and dot blot analyses demonstrate miR-122 expression in both transfected HeLa cells and murine hepatocytes, but miR-122 expression was not found in HeLa cells transfected with pAVU6+27. Conclusion The expression of miR-122 in HeLa cells may provide the basis for further studies of its targets and biological functions.
