Quantitative analysis of IGF1Rs on human hepatocellular HepG_2 cells
- VernacularTitle:HepG_2人肝癌细胞胰岛素样生长因子1受体定量分析
- Author:
Chaoxue LUO
;
Shaojun CHENG
;
Qianwei LI
- Publication Type:Journal Article
- Keywords:
HepG 2 cells;
IGF1R;
quantitative analysis
- From:Journal of Third Military Medical University
1983;0(03):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To quantitatively analyze type 1 receptor of insulin-like growth factors (IGF1Rs) on the HepG 2 cells. Methods 1H7 (monoclonal antibody against IGF1R) was labeled with radionuclide 131 I by Iodogen method. The mixture was purified with Sephadex G-50 column, 131 I-1H7, and its unlabeled counterpart 1H7 competitively bound to IGF1Rs on the HepG 2 cells. The average number of IGF1R on the HepG 2 cells was analyzed by Scatchard method. Results The Bmax of HepG 2 cells (2.0?10 5) in every well was 6.746?10 -12 mol/L. The expression of IGF1R on the HepG 2 cells was 2.03?10 4/cell. Conclusion IGF1Rs are expressed on the HepG 2 cells with high density and high affinity, which may be used in other related studies.
