PCR-RFLP analysis of mitochondrial DNA control region in patients with osteosarcoma
- VernacularTitle:人骨肉瘤组织线粒体DNA控制区PCR-RFLP分析
- Author:
Xueguang GUO
;
Qiaonan GUO
- Publication Type:Journal Article
- Keywords:
osteosarcoma;
mitochondrial DNA;
PCR-RFLP;
mutation
- From:Journal of Third Military Medical University
1983;0(03):-
- CountryChina
- Language:Chinese
-
Abstract:
0.05) found between the three subtypes of osteosarcoma with rates of the A deletion being 30% (3/10), 40% (2/5), and 40% (2/5) in three subtypes, respectively. The T16519C mutation and the A deletion in the nucleotide position 16 132 (5′ end) were co-detected in 4 (20%) out of 20 samples. The levels of the two mutations in patients with osteosarcoma were significantly higher than those in healthy human. Conclusion The two mutations have high rates in osteosarcoma and may play an important role in carcinogenesis and progression of osteosarcoma.
