Application of amplified fragment length polymorphism in the study of genetic diversity of Oncomelania hupensis
- VernacularTitle:AFLP标记技术在湖北钉螺遗传多样性中的应用研究
- Author:
Yibiao ZHOU
;
Qingwu JIANG
;
Genming ZHAO
- Publication Type:Journal Article
- Keywords:
Oncomelania hupensis;
Amplified fragment length polymorphism;
Genetic diversity
- From:
Chinese Journal of Schistosomiasis Control
1989;0(01):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To explore the application of amplified fragment length polymorphism (AFLP) in the study of genetic diversity of Oncomelania hupensis. Methods A snail was taken at random from Dali region, Yunnan Province and Yueyang City, Hunan Province respectively, and DNA was drawn with guanidinium thiocyanate and resin et al. Genomic DNA was amplified selectively by the AFLP technique with 64 pairs of primers, and the polymorphism of PCR products was examined by SDS-PAGE. Results The number of AFLP markers was between 5 and 55 for a pair of primers. The average number of a pair of primers was 38.30 (95% CI 36.03-40.57) for the snail from Yunnan Province, and 39.14 (95% CI 36.71-41.57) for the one from Hunan Province. For a pair of primers, the number of amplified polymorphic markers was between 3 and 37, and the polymorphic rate was between 28.6% and 76.2%, the average, 23.67 (95% CI 22.12-25.22) and 47.36%(95 %CI 45.22%-49.50%), respectively. The average similarity between the two populations was 0.69 (95% CI 0.67-0.70). Conclusion The AFLP technique is a new path for classifying Oncomelania hupensis and studying the genetic diversity of it.[
