Anti-inflammatory and Anti-oxidant Effects of Sophora flavescens Root Extraction in Lipopolysaccharide-activated Raw 264.7 Cells.
- Author:
Dong Hoon LEE
1
;
Dong Suep SOHN
;
Dai Yun CHO
;
Beom Joon KIM
;
Yun Young LIM
;
Young Heui KIM
Author Information
1. Department of Emergency Medicine, Chung-Ang University College of Medicine, Seoul, Korea.
- Publication Type:Original Article
- Keywords:
Sophora flavescens;
iNOS;
COX-2
- MeSH:
Antioxidants;
Cyclooxygenase 2;
Dinoprostone;
Enzyme-Linked Immunosorbent Assay;
Free Radicals;
Inflammation;
Interferon-gamma;
Interleukin-6;
Macrophages;
Medicine, East Asian Traditional;
Medicine, Traditional;
NF-kappa B;
Nitric Oxide Synthase Type II;
Proteins;
Sophora
- From:Korean Journal of Medical Mycology
2010;15(2):39-50
- CountryRepublic of Korea
- Language:Korean
-
Abstract:
BACKGROUND: The macrophages activated by lipopolisaccharide produce numerous molecules and proteins, such as tumor necrotic factor-alpha (TNF-alpha), interleukin-6 (IL-6), IL-1beta, inducible nitric oxide synthase (iNOS), cyclooxygenase-2 (COX-2), and free radicals, associated with inflammation. The response was occurred by intracellular cascaded, NF-kappaB pathway. So, the regulation of this pathway is very important in control of inflammation. OBJECTIVE: In this study, the anti-inflammatory and anti-oxidant effects of Sophora flavescens that is used empirically in oriental medicine and folk remedy were evaluated and the mechanism of the effects was studied. METHODS: By using the root extracts of Sophora flavescens, we performed experiment in LPS and interferon-gamma (IFN-gamma)-activated Raw 264.7 cells. We measured the production of NO, PGE2 and expression of iNOS and COX-2 in activated Raw 264.7 cells with Sophora flavescens root extract. Also, we tested anti-oxidant effect of Sophora flavescens root extracts by ELISA kit in activated Raw 264.7 cells, and the free radical scavenging effect of material itself by DPPH assay. RESULTS: The Sophora flavescens root extracts decreased the production of NO (p<0.001) and PGE2 (p<0.01) in Raw 264.7 cells activated by LPS and IFN-gamma. The expression of proteins, iNOS and COX-2, suppressed along with the elevated concentration of Sophora flavescens root extracts. The result of DPPH assay was that the test material itself had scavenging effect for free radical (p<0.001). And the antioxidant activity in activated Raw 264.7 cells was increased with the level of the Sophora flavescens root extracts (p<0.05). CONCLUSION: The Sophora flavescens root extracts suppressed the production of NO and PGE2 through the decreased expression of iNOS and COX-2. And the Sophora flavescens root extracts had the scavenging effect about free radicals itself and increased the antioxidant activity in activated macrophages.
