Effects of different dosage of recombinant human erythropoietin on pulmonary angiogenesis in newborn rats exposed to hyperoxia
10.3760/cma.j.issn.1673-4912.2012.03.017
- VernacularTitle:不同剂量促红细胞生成素对新生大鼠高氧肺损伤血管保护作用的研究
- Author:
Yanmin BAO
;
Shoujin KUANG
;
Zuocheng YANG
- Publication Type:Journal Article
- Keywords:
Recombinant human erythropoietin;
Hyperoxia;
Lung injury;
Rat,newborn
- From:
Chinese Pediatric Emergency Medicine
2012;19(3):272-275,278
- CountryChina
- Language:Chinese
-
Abstract:
ObjectiveTo investigate the effect of different dosage recombinant human erythropoietin(rhEPO),an angiogenesis-like factor,on pulmonary angiogenesis exposed to hyperoxia in newborn rats.MethodsSixty Sprague-Dawley newborn rats were randomly divided into four groups:air group (room air exposure,n =15 ),hyperoxia group ( exposed to 95％ oxygen,n =15 ),hyperoxia + large dosage rhEPO group (received rhEPO 5000 U/kg,intraperitoneally on 1 hour before and 3 days after exposed to hyperoxia,n =15) and hyperoxia + small dosage rhEPO group (received rhEPO 800U/kg,the same time points,n =15 ).The isodose of saline were given intraperitoneally on the same time points in the air group and the hyperoxia group.After 6 d of exposure,the survival rate was compared,CD31 and vascular endothelial growth factor (VEGF) were measured by immunohistochemistry to assess hyperoxia-induced changes in lung morphology.ResultsAfter 6d of exposure,hyperoxia + large dosage rhEPO group prolonged the survival rate in comparison with the hyperoxia group [ 86.7 ％ ( 13/15 ) vs 60.0 ％ ( 9/15 ) ].The expression of lung CD31 [ ( 38.69 ±1.69)％ vs (33.57±4.12)％,P＜0.05] and VEGF (124.4296±7.2823 vs 114.2059 ±-8.345 7,P＜0.05) in newborn rats treated with large dosage of rhEPO was significantly higher than those in hyperoxia group.While there was no significant difference of CD31 [ ( 36.34 ± 1.89 ) ％ ] and VEGF( 115.429 6 ± 6.719 9) in small dosage rhEPO group compared with the hyperoxia group (P＞0.05 ).ConclusionInstead of treatment with small dosage rhEPO (800 U/kg),large dosage rhEPO (5000 U/kg) may have important protective effects on pulmonary angiogenesis in hyperoxia-induced lung injury of newborn rats.
