Experimental study on the expression of Cx43 protein in normal ciliary body and traumatic aPVR hypotony in cat
- VernacularTitle:猫睫状体及外伤后aPVR慢性低眼压模型中Cx43的表达
- Author:
Ying ZHANG
;
Maonian ZHANG
- Publication Type:Journal Article
- Keywords:
ciliary body;
aPVR;
Cx43;
immunohistochemistry
- From:
Medical Journal of Chinese People's Liberation Army
1983;0(02):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the expression of gap junction(GJ) protein Cx43 in normal cat ciliary body and its corresponding changes in location and quantity in traumatic anterior proliferative vitreoretinopathy (aPVR) model at different time points and under different intraocular pressure (IOP). Methods In a cat model of chronic hypotony after traumatic aPVR IOP was measured before and after trauma. Similar measurement was done in healthy cats. The location and content of Cx43 of ciliary epithelium was determined with immunohistochemistry technique separately in normal group and 24h, 3d, 1w, 2w and 4w after trauma in the experimental group. Results Cx43 was located between the bi-epithelium of ciliary body of the normal cat, forming GJ to communicate between PE and NPE. However, in the aPVR model, Cx43 appeared between PE cells also, and was up-regulated obviously at 24h, At 1w, Cx43 was seen to be distributed diffusely between all epithelial cells reaching the maximum level. Its level descended gradually after 2w and 4w. The IOP raised slightly after 24h and then gradually fall. The changes in Cx43 quantity was basically parallel to ?IOP within 3d and after 1w. Conclusion In cat ciliary body Cx43 from the direct transportation channels for aqueous materials between PE and NPE. Its changes in location and quantity bore close relation ship with the formation of hypotony after aPVR.
