Construction of EGFP-labled recombinant plasmid of VEGF165 gene and its expression in bone marrow mesenchymal stem cells
- VernacularTitle:EGFP标记的VEGF165重组质粒的构建及其在骨髓间充质干细胞中的表达
- Author:
Fu YI
;
Rongqing ZHANG
;
Guoliang JIA
- Publication Type:Journal Article
- Keywords:
endothelial growth factor;
fluorescent antibody technique;
plasmid;
stem cells
- From:
Medical Journal of Chinese People's Liberation Army
1983;0(02):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To construct a EGFP-labled recombinant plasmid of VEGF165 (vascular endothelial growth factor) gene, and to study the transfection and expression of VEGF165 eukaryotic expression plasmid in mesenchymal stem cells (MSCs). Methods pIRES2-EGFP-VEGF165 recombinant plasmid was constructed, which was then transfected into rat MSCs. ELISA and MTT were used to detect the expression level and biological activity of VEGF in the conditioned medium after transfection. Results There was a significant increase in VEGF protein in the MSCs after being transfected with pIRES2-EGFP-VEGF165. The conditioned medium after transfection showed the biological activity of stimulating the proliferation of endothelial cells. Conclusions The pIRES2-EGFP-VEGF165, a eukaryotic expression plasmid for VEGF165 gene, is constructed. High levels of VEGF protein expression can be obtained in the MSCs transfected with pIRES2-EGFP-VEGF165. The expressed protein has the biological activity of VEGF.
