An experimental model for alginate immune pneumonia in rabbits
- VernacularTitle:藻酸盐免疫性肺炎家兔模型的建立
- Author:
Hongyan LI
;
Qianming XIA
;
Keshu LUO
- Publication Type:Journal Article
- Keywords:
alginates;
immunity;
pneumonia;
disease model, animal
- From:
Medical Journal of Chinese People's Liberation Army
1983;0(02):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To reproduce a rabbit model of alginate immune pneumonia, and to study the pathogenic role of alginate on lung tissue. Methods Rabbits of group A, being challenged by saline, served as the control group. Rabbits of group B and C were immunized with seaweed alginate and bacterial alginate respectively. Body temperature,peripheral blood cells and the serum level of circulating immune complex (CIC) were measured after the challenge. Bronchoalveolar lavage fluid (BALF) specimens were incubated and bacterial colonies were counted. The cell number and differential count of BALF were made. The pathological changes of the lung tissue were observed under microscopy. Results In both seaweed alginate and bacterial alginate groups, the peripheral blood cell count and the number of bacterial colonies in BALF were similar to that of the saline group. However, the serum CIC levels and the cell count of BALF were increased significantly after the challenge. Under light microscopy, lymphocyte infiltration and lymphoid follicle formation around the bronchiolus were found in all alginate immunized groups, and there was also narrowing of bronchioli. Conclusion Both seaweed alginate and bacterial alginate could be used to establish the model of alginate immune pneumonia in rabbits. Alginate can cause inflammatory reaction and pathological changes in the rabbit lung, manifesting mainly peribronchilar lymphocytic infiltration and lymphoid follicle formation around the bronchioli.
