Effects of aldosterone on nos-no system activity of cardiac fibroblasts in neonatal rat
- VernacularTitle:醛固酮对鼠心肌成纤维细胞NOS-NO系统活性的影响
- Author:
Huiqing FENG
;
Jinkai WEI
;
Huizhe LIU
- Publication Type:Journal Article
- Keywords:
aldosterone;
nitricoxide;
nitric oxide synthase;
cardiac fibroblasts
- From:
Medical Journal of Chinese People's Liberation Army
1983;0(02):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the changes in nitric oxide synthase (NOS) activity in cardiac fibroblasts (CFs) of neonatal rat induced by aldosterone. Method CFs were isolated by trypsin digestion method. Proliferation of CF was assessed by MTT colorimetric assay. Nitric acid reductase method and spectrophotometry were used to determine the NO contents and NOS activity, respectively, at different time after CFs being treated with aldosterone. Result Aldosterone (1?10 -11 -1?10 -7 mol/L) could increase CFs proliferation in a dose-dependent manner. NO contents, NOS activity and iNOS mRNA were decreased in a dose-dependent manner after being treated with aldosterone in different concentrations (P
