Expression of G-CSFR and its mRNA in human colorectal cancer and normal mucosa tissues
- VernacularTitle:G-CSF受体及其mRNA在结直肠癌和其附近正常黏膜中的表达
- Author:
Xiaodong YANG
;
Fukun LIU
;
Zhe XU
- Publication Type:Journal Article
- Keywords:
receptors, granulocyte colony-stimulating factor;
colorectal;
immunohistochemistry;
reverse transcriptase polymerase chain reaction
- From:
Medical Journal of Chinese People's Liberation Army
1983;0(02):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective The purpose of the study was to study the expression of granulocyte colony-stimulating factor receptor (G-CSFR) in human colorectal cancer (CPC) and the role of granulocyte colony-stimulating factor (G-CSF) in the development and progression of human CRC. Methods Forty-two specimens of CRC and normal colorectal mucosa were harvested from colon or rectum in a group of patients who were suffering from CRC. Immunohistochemistry (IHC) and reverse transcription-polymerase chain reaction (RT-PCR) technique were used to demonstrate expression of granulocyte colony-stimulating factor receptor. The relationship between expression of G-CSFR and clinical or pathological pictures was analyzed. Results Immucohistochemical analyses revealed that G-CSFR was expressed in the human colorectal cancer (25/42, 59.52%), and it appeared to be up-regulated compared with the normal mucosa (14/42, 33.33%, P
