Fermentation technology in heat-labile enterotoxin B subunit of genetic recombinant E. coli
- VernacularTitle:大肠杆菌不耐热肠毒素B亚单位基因工程菌发酵工艺的研究
- Author:
Wenbiao TIAN
;
Quanming ZOU
;
Weijun ZHANG
- Publication Type:Journal Article
- Keywords:
enterotoxigenic E.coli;
heat-labile enterotoxin B subunit;
fermentation
- From:Journal of Third Military Medical University
1984;0(02):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the fermentation conditions for the heat-labile enterotoxin B subunit (LTB) of genetic recombinant E.coli. Methods The effects of different kinds of media, the range of pH, the induction time, the glucose concentration, and the fed-batch on LTB level in 10-liter fermenter under the condition of cascade controlling dissolved oxygen content were analyzed. Results Under the established conditions, the cell output per liter was 40.5g, and the expression level was 30.6%. Conclusion The results indicate that the stable fermentation technology with high efficiency has been established.
