Expression of inositol 1,4,5-triphosphate receptor in airway smooth muscle cells of rats and its roles in chronic asthma formation
- VernacularTitle:三磷酸肌醇受体各亚型在大鼠气道平滑肌细胞中的表达以及在慢性哮喘中的作用
- Author:
Ying WANG
;
Suiyang ZHANG
;
Xiliang WANG
;
Guishen QIAN
- Publication Type:Journal Article
- Keywords:
inositol 1,4,5-triphosphate;
receptor;
airway smooth muscle;
reverse transcriptase-polymerase chain reaction;
rat
- From:Journal of Third Military Medical University
1984;0(02):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To detect the expression of inositol 1,4,5-triphosphate receptor (IP 3R) subtypes in normal rat airway smooth muscle cells (ASMCs) and changes during chronic asthma formation. Methods ASMCs were cultured by collagen enzyme digestion method. The expressions of subtypes of IP 3R were detected by RT-PCR and the purified PCR products were linked with pGEM-T vector for DNA sequencing. Chronic asthma model was established with egg albumin. The changes of IP 3Rs were detected by RT-PCR method. Results All subtypes of IP 3R were expressed in airway smooth muscle cells of normal rats. The expression of IP 3R1 in asthma groups increased obviously as compared with that in the control group (P
