Preparation of a new cationic liposome and determination of its gene transfection rate
- VernacularTitle:新型阳离子脂质体的制备及其转染效率的测定
- Author:
Han LEI
;
Guisheng QIAN
;
Guoming WU
;
Guijun HUANG
- Publication Type:Journal Article
- Keywords:
N 4-spermine cholesteryl carbamate;
cationic liposome;
cytofectin;
gene delivery vehicle;
gene transfection
- From:Journal of Third Military Medical University
1984;0(02):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To preparation a new cationic liposome of which the positive component is a new cytofectin as a gene delivery vehicle for laboratory research of gene transfection and gene therapy in lung diseases. Methods The new cationic liposome was prepared by film. Electron microscopy and gel electrophoresis were employed to define the condition for transfection. The prepared cationic liposome was used in the transfection of a fluorescence plasmid to lung cancer cell line SPC and the rate of the transfection was evaluated. Results The prepared new cationic liposome was global microcapsule in size of 100 nm-1 ?m. The highest transfection rate of 60% was attained when the liposome was in a ratio of (6-8)∶1 with plasmid. Conclusion The new cationic liposome is prepared with cytofectin N 4-spermine cholesteryl carbamate as its positive component, and sound transfection activity is observed under a certain circumstance. It provides a basis for gene transfection and gene therapy.
