Culture and evaluation of rat’s mesenteric lymphatic endothelium in vitro
- VernacularTitle:大鼠肠系膜淋巴管内皮细胞体外培养方法的建立及鉴定
- Author:
Zhimin DU
;
Ye LIU
;
Baofeng YANG
- Publication Type:Journal Article
- Keywords:
mesentery;
lymphatic;
endothelium;
cell culture;
NOS;
fac tor Ⅷ related antigens
- From:
Chinese Pharmacological Bulletin
1987;0(01):-
- CountryChina
- Language:Chinese
-
Abstract:
Aim To establish the technology of isolation rat’s mesenteri c lymphatics and the culture method of their endothelium and confirm the ability o f synthesis nitric oxide and Factor Ⅷ related antigens in the endothelium in vitro. Methods With an operating microscope and microsurgical in struments, rat’s mesenteric lymphatics were isolated and the endothelium were p rimarily cultured and subcultured. Exerting immunohistochemical method, Factor Ⅷ related antigens and nitric oxide synthase were evaluationed. The contents of NO metabolite were measured. Results The rat’s mesenteric lympha tics were isolated and the endothelium were Fusiform shape or polygon, the monol ayer cultures displayed typical cobblestone or pavingstone morphology. Factor Ⅷ related antigens were expressed and their positive rate was 98%. The positive rate of nitric oxide synthase was 83%. Positive reaction products were buffy ar ound nuclear. NO metabolite was (10.26?2.17) mol?L -1(n=7). Conclusion The culture method we introduced was very practical, simple and reliable. The endothelium is in normal structure and function characters. It also certificated that the endothelium in vitro could express nitric oxide synthase and Factor Ⅷ related antigens.
