Role of BMP-7 expression in renal tubulointerstitial lesions induced by unilateral ureteral obstruction
- VernacularTitle:骨成形蛋白-7在大鼠肾小管间质损害中的作用
- Author:
Jianfeng ZHOU
;
Na LI
;
Yaoquan ZHANG
;
Fahuan YUAN
- Publication Type:Journal Article
- Keywords:
Bone morphogenetic protein-7;
Unilateral ureteral obstruction;
Fibrosis
- From:
Chinese Journal of Nephrology
1997;0(01):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To explore the role of bone morphorgenetic protein-7(BMP-7) in the renal tubulointerstitial lesions induced by unilateral ureteral obstruction (UUO).Methods After dividing into normal control, sham operation and unilateral ureteral obstruction (UUO) groups, sixty rats were sacrificed at postoperative day 1, 3, 7, 14. The levels of BMP-7 and TGF-?1 mRNA were examined by RT-PCR. The sites and levels of protein expression of BMP-7, TGF-?1 and ?-SMA were detected by immunohistochemistry staining. Results Compared to control groups, BMP-7 mRNA was significantly decreased, but TGF-?1 mRNA was significantly increased in UUO rats. Immunohistochemistry staining studies indicated that BMP-7 mainly expressed in renal tubule and interstitium, rarely in glomeruli. In UUO rats, the protein expression of BMP-7 decreased, but the expression of TGF-?1 and ?-SMA increased in varying degrees according to the obstructed days. The renal tubulointerstitial expression of BMP-7 was significantly negatively correlated with the expression of TGF-?1 and ?-SMA. Conclusion The loss of BMP-7 is explored in the early phase of fibrotic process and may play a very important role in mediating the tubulointerstital lesions.
