Roles of bone marrow-derived stem cells in regeneration of renal tubules after renal ischemia-reperfusion injury in mice
- VernacularTitle:小鼠骨髓源性干细胞迁移到缺血再灌注损伤肾脏内表达CK18和RCA的观察
- Author:
Jinglan DAI
;
Benlan YE
- Publication Type:Journal Article
- Keywords:
bone marrow-derived stem cell;
ischemia reperfusion injury;
renal tubular epithelial cell
- From:Journal of Third Military Medical University
1984;0(01):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To test whether murine bone marrow-derived stem cells (BMSCs) can contribute to the regeneration of renal tubular epithelial cells after ischemia-reperfusion (I/R) injury. Methods The female mice with bilateral I/R injured kidney received transplantation of bone marrow mononuclear cells isolated from homologous male mice via the tail vein after sublethal irradiation. After bone marrow transplantation (BMT), Sry expressions in the bone marrow of the recipient mice were detected by PCR. Serial-section analysis of Y chromosome fluorescence in situ hybridization and immunohistochemistry with CK18 or RCA were performed to observe the differentiation of bone marrow-derived stem cells in the I/R injured kidney. Results At 15 d after BMT, PCR analysis confirmed the presence of Sry gene expression in the bone marrow of the recipient mice. AT 30 d after BMT, Y +/CK18 + and Y +/RCA + cells were detected in the renal tubules of the recipients. Conclusion The microenvironment of the injured kidney can induce BMSCs to differentiate into renal tubular epithelial cells which express CK18 or RCA and participate in the regeneration of the injured renal tubule. The results suggest the clinical potential of BMSCs in the cell replacement therapy for renal failure.
