Construction of transmembrane TNF-? eukaryotic expression vector in n-3 PUFA dependant manner
- VernacularTitle:n-3 PUFA依赖的跨膜型肿瘤坏死因子-?真核表达载体的构建
- Author:
Hai CHENG
;
Mantian MI
;
Qianyong ZHANG
;
Da CHEN
- Publication Type:Journal Article
- Keywords:
n-3 PUFA;
transmembrane TNF-?;
peroxisome proliferator response element;
eukaryotic expression
- From:Journal of Third Military Medical University
1984;0(01):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To construct the transmembrane TNF-? eukaryotic expression vector in n-3 PUFA dependant manner. Methods PPRE-tk sequence was designed and artificially synthesized, and then inserted it into pcDNA3-TNF( ? 1-12)WB plasmid to construct eukaryotic expression vector pPPRE-tk-tmTNF-? by gene recombine techniques. The tmTNF-? protein expression level was observed in MCF-7 transfected cells incubated with EPA by immunofluorescence technique. Results pPPRE-tk-tmTNF-? expression vector was constructed successfully and identified by agarose gel electrophoretic analysis and nucleotide sequence analysis. EPA could increase tmTNF expression levels in time- and dose-dependant manners. Conclusion tmTNF-? expression vector regulated by n-3 PUFA is successfully constructed.
