Establishment and identification of tumor fibroblast model
- VernacularTitle:肿瘤基质成纤维细胞模型的建立及生物学特性研究
- Author:
Hongmei ZHU
;
Weixue TANG
;
Weiping ZHOU
;
Xiaoyan ZHANG
;
Jing MA
;
Weipin ZHENG
- Publication Type:Journal Article
- Keywords:
tumor;
fibroblast;
cell model;
L 929 -H 22
- From:Journal of Third Military Medical University
1984;0(01):-
- CountryChina
- Language:Chinese
-
Abstract:
Objectives To establish the cell model of fibroblast in tumor and explore its malignant bionomics for providing valuable cell model for studying their transforming mechanism and developing drugs targeting the tumor stroma in the future. Methods The murine normal fibroblast cells, L 929 , were cultured with the supernatant of murine hepatocellular carcinoma cells, H 22 , for three and a half months, and L 929 -H 22 cells were acquired with stable growth. Then the changes of their biological characteristics were determined by light microscopy, electron microscopy, karyotype analysis, and MTT assay. The expressions of PCNA, bcl-2, MMP-9, TN, and tolomerase activity were detected by immunocytochemistry and RT-PCR, respectively. The ability to synthesize proteins and the effects of their supernatant on H 22 growth were determined. Results Abnormal nuclei and multinuclei were observed in L 929 -H 22 cells, and their chromatosome modes increased slightly. As compared with the parental cells, the population doubling time of L 929 -H 22 cells shortened (t=2.654 1, P
