Effect of PKC antagonist-chelerythrine on mouse pancreatic secretion stimulated by cholecystokinin
- VernacularTitle:PKC拮抗剂对缩胆囊素刺激下的小鼠胰腺腺泡淀粉酶分泌的影响
- Author:
Zhibo DENG
- Publication Type:Journal Article
- Keywords:
Pancreatic acini Cholecystokinin Carbachol Protein kinase C Chelerythrine
- From:
Chinese Journal of Practical Internal Medicine
2003;0(01):-
- CountryChina
- Language:Chinese
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Abstract:
Objective To establish a mouse pancreatic exocrine secretion system,in order to study the effect of secretagogues,such as cholecystokinin (CCK) and carbachol (CCH),on the exocrine secretion of mouse pancreas,and use a protein kinase C (PKC) antagonist-chelerythrine(Che) to research the possible mechanism.Methods Prospective control study.Results CCK and CCH increased the secretion of amylase from the mouse pancreatic acini about two fold of that of the control (7.02%vs14.29%),and,CCK or CCH added Che,could increase the secretion of amylase to a level of about three fold(19.02%).Conclusion CCK or CCH stimulate the pancreatic secretion concerning the PKC cellular signal system;surprisingly,a PKC antagonist,chelerythrine,can reinforces their stimulation.
