Expression of MIP-3? gene regulated by irradiation via Egr-1 promotor in Lewis lung cancer
- VernacularTitle:小鼠MIP-3?基因在Lewis肺癌中的放射诱导表达
- Author:
Lan ZOU
;
Bo ZHU
;
Jing CHEN
;
Jianping DU
;
Zhengtan CHEN
- Publication Type:Journal Article
- Keywords:
macrophage inflammatory protein 3?;
early growth response-1 promoter;
dendritc cell;
radio-genetic therapy
- From:Journal of Third Military Medical University
2003;0(24):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To construct the expression vector of early growth response-1 promoter-macrophage inflammatory protein 3? (pEgr-1-MIP-3?, pEM) to study MIP-3? expression in transfected Lewis lung cancer cells. Methods MIP-3? cDNA was inserted into plasmid with Egr-1 promoter by directional cloning. The vectors were transfected into Lewis lung cancer cells with liposomes. The expression of MIP-3? in transfected Lewis lung cancer cells induced by electron ray irradiation at different doses were confirmed by RT-PCR and Western blotting. Results The recombinant vector was sequenced, and the result indicated the correct insertion in the expression vector. After electron ray irradiation at different doses, MIP-3? expression in the transfected Lewis lung cancer cells increased significantly. Conclusion Electron ray can enhance the expression of MIP-3? in Lewis lung cancer cells. This has laid the experimental foundation for the studies of pEM for radio-genetic therapy in vivo.
