Isolation, purification and identification of nucleosomes from chicken erythrocytes
- VernacularTitle:核小体的提取纯化及鉴定
- Author:
Baiyu ZHONG
;
Jin HAO
;
Fei HAO
;
Yunsheng XU
- Publication Type:Journal Article
- Keywords:
nucleosome;
isolation;
purification;
identification
- From:Journal of Third Military Medical University
2003;0(24):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To establish a simple and practical method for isolating and purifying nucleosomes. Methods Nuclei were isolated from chicken erythrocytes, and then digested with staphylococcal nuclease. After centrifugation, the supernatant of digestion was separated and centrifugated on sucrose gradients. Results Nucleosomes with good stability were isolated properly by gradient centrifugation. Conclusion This method for the isolation and purification of nucleosomes is simple and effective, which might contribute to the further researches of the roles of nucleosomes in the pathogenesis of systemic lupus erythematosus.
