Study on activity and mechanism of baicalin against candida albicans
- VernacularTitle:黄芩苷抗白念珠菌作用及机制研究
- Author:
Ying XIONG
;
Yingyuan FU
;
Nanzhen KUANG
;
Wenping ZHANG
- Publication Type:Journal Article
- Keywords:
baicalin;
candida albicans;
drug effect;
isotope-incorporation
- From:
Chinese Pharmacological Bulletin
2003;0(12):-
- CountryChina
- Language:Chinese
-
Abstract:
Aime To study the anti-C. albicans activity of baicablin,and to explore its anti-C.albicans mechanism preliminarily. Method ① The minimum inhibitory concentration (MIC) and the minimum fungicidal concentration(MFC) of baicalin against C.albicans were determined by microdilution method. ② By means of incorporation experiment of isotope-labelled precursor, the incorporation inhibitation rates of [ 3H]-TdR,[ 3H]-UdR,[ 3H]-leucine were measured after treatment with various concentrations of baicalin respectively. Result ① The MIC and MFC of baicalin (above purity 98%) against C albicans(1?107 cfu?L -1) were both 1.0 g?L -1. The MIC and MFC of baicalin (above 98% purity) against C albicans(2.5?107 cfu?L -1) both 2.0 g?L -1. ② The dose of baicalin of 0.5, 1 and 2 MIC could obviously inhibit incorporation of the precursors and there were significant differences among the inhibition rate of three precursors incorporation (P0.05). Conclusion ① Baicalin had strong anti-C albicans activity in vitro. ② Baicalin(above purity 98%) could obviously inhibit incorporation of [ 3H]-TdR, [ 3H]-UdR and [ 3H]-leucine into C albicans and the incorporation inhibition rate of [ 3H]-UdR was highest among them, suggesting that the anti-C albicans mechanism of baicalin may be involved with the inhibition of DNA, RNA and protein synthesis.
