Rapid detection of Amp C ?-Iactamase in Enterobacter cloacae by PCR
- VernacularTitle:基因扩增法快速检测阴沟肠杆菌Amp C ?-内酰胺酶
- Author:
Hu ZHAO
;
Mingjun HE
;
Shiliang ZHANG
- Publication Type:Journal Article
- Keywords:
Amp C ?-lactamase;
PCR;
E. cloacae
- From:
Academic Journal of Second Military Medical University
1999;0(12):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To establish a new method to detect Amp C?-lactamase by PCR. Methods:The amp C and amp D gene fragments of E. cloacae were amplified by the amp C and amp D primers to detect Amp C ?-lactamase, especially the enduring and highly productive enzymes. Results:Totally 193 of 214 strains of E. cloacae were positive for amp C gene , implicating most strains of E. cloacae had the ability to produce the enzyme. Sixteen of the 193 strains (amp C positive) were negative for amp D genes, implicating these 16 strains could produce the enduring and highly productive enzymes. The results were confirmed by cefoxitin three-dimensional test. Conclusion:The new method to detect Amp C ?-lactamase, especially the enduring and highly productive enzymes,by amp C and amp D primers is a rapid and accurate method.
