Doranidazole in the radiosensitization of hypoxic pancreatic cancer cells
- VernacularTitle:Doranidazole对乏氧胰腺癌细胞放射增敏研究
- Author:
Li ZHANG
;
Shulian LI
;
Jun JI
- Publication Type:Journal Article
- Keywords:
Cell line,pancreatic tumor;
Cell hypoxia;
Radiation-sensitizing agent;
Cell death
- From:
Chinese Journal of Radiation Oncology
1992;0(04):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the effects of a new radiosensitizer, nitroimidazole-derivative--Doranidazole on pancreatic cancer cells under hypoxic condition. Methods The human pancreatic cancer cell lines were exposed in vitro to a single fraction of high dose?-ray radiation either with doranidazole or under hypoxic condition. The percentage of dead cells was analyzed with a multiwell plated reader and the fluorescence intensities of propidium iodide before and after a digitonin tratment were measured. The sensitizing effect of doranidazole on cell killing by high-dose irradiation was evaluated by time-course, dose-dependency, and microscopic observations. The selective radiosensitive effect of doranidazole on hypoxic cells was evaluated by flow cytometry. Results Of seven pancreatic cancer cell lines, the mortalities of 5 cell lines were significantly increased in presence of doranidazole under hypoxic condition 5 days after 30 ?Gy irradiation, but not under aerobic condition. Meanwhile, the radiosensitizing effect of Doranidazole on pancreatic cancer cells was demonstrated as time- and dose-dependent manner. Conclusion Doranidazole can enhance the radiosensitivity on pancreatic cancer cells under hypoxic condition with high-dose irradiation.
