STUDY OF THE SYNERGETIC EFFECT OF FOLIC ACID AND GENISTEIN ON THE EARLY STAGE OF PRIMARY CULTURED NEURON APOPTOSIS
- VernacularTitle:叶酸配伍染料木黄酮对神经细胞早期凋亡的影响
- Author:
Rong XIAO
;
Jiang LIANG
;
Haifeng ZHAO
- Publication Type:Journal Article
- Keywords:
folic acid;
genistein;
neuron apoptosis
- From:
Acta Nutrimenta Sinica
2004;0(06):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective: To observe the synergetic effect of folic acid and genistein on apoptosis in the early stage of neuron and its possible mechanism. Methods: Cyclophosphamide was added to primary cultured neurons to induce apoptosis. Three different dosages of folic acid and genistein were used to inhibit apoptosis. The results were observed by flow cytometry and scanning electron microscopy (SEM). Results: 1.Folic acid and genistein might play a role in early stages of neuron apoptosis, and the synergetic effect was demonstrated when folic acid and genistein were administered together. 2. The membrane changes in early stages of neuron apoptosis observed by scanning electron microscope were also in agreement with the flow cytometry data. Conclusion: Genistein might reinforce the protective effect of folic acid on neural tube defects by restraining the apoptosis of the early stage of neuron.
