The cryopreservation of human oocytes at different maturity stages
- VernacularTitle:不同成熟期人卵母细胞慢速冷冻的初步研究
- Author:
Zijiang CHEN
- Publication Type:Journal Article
- Keywords:
Oocytes/ultrastruct;
Cryopreservation;
Fertilization;
in vitro
- From:
Journal of Peking University(Health Sciences)
2003;0(06):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To examine the ultrastructure changes and the effects ot different sucrose con-centrations on the developmental potential of human frozen-thawed oocytes at different maturity stages.Methods:Oocytes at different maturity stages collected from polycystic ovarian syndrome patients wereinvolved in the study.Different sucrose concentrations(0.1,0.2 or 0.3 mol/L)were used to study thedevelopmental potential of the frozen-thawed oocytes.Non-frozen and frozen-thawed different maturity oo-cytes were processed for transmission electron microscopy observation.Results:The results revealed thatthe mitochondria and the vesicles in the immature oocytes cytoplasm were fewer than those in the matureoocytes without regular distribution.Electron density of the mitochon dria and distribution of the vesiclesin the mature oocytes changed with the cryopreservation.No remarkable change was produced in the im-mature oocytes between non-frozen and frozen-thawed oocytes.Cryopreserving with 0.2 mol/L sucrose re-sulted in perfect development potential for both mature and immature oocytes than that with 0.1 mol/L.Study involving 0.3 mol/L sucrose in the cryoprotectant resulted in higher survival rate and clinical preg-nancies.Conclusion:The results suggest that sucrose concentration of 0.3 mol/L in the cryoprotectantsolution is efficient in freezing oocytes with slow-freezing method.Cryopreservation with slow-freezingmethod could produce ultrastructure changes in the human oocytes.
