A two-dimensional reference map of mouse ovary proteins
- VernacularTitle:小鼠卵巢蛋白质双向凝胶电泳蛋白谱系的初步建立及其功能分析
- Author:
Xiang MA
- Publication Type:Journal Article
- Keywords:
Proteins/anal;
Ovary;
Electrophoresis;
gel;
two-dimensional;
Spectrum analysis;
mass;
Mice;
inbred ICR
- From:
Journal of Peking University(Health Sciences)
2003;0(06):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To perform a preliminary proteomic analysis of mouse ovaries and to study theprotein's function in mouse ovary.Methods:The two-dimensional gel electrophoresis(2-DE)and matrixassisted laser desorption/ionization-time of flight mass spectrometry( MALDI-TOF MS)were used to ana-lyze mouse ovarian proteome.A 12.5% sodium dodecyl sulfate(SDS)reference gel was generated byimmobilized pH gradient isoelectric focusing of mouse ovary proteins in a non-linear gradient(pH 3-10).And GRP78 was selected to perform with immunohistochemistry within mouse ovaries.Results:Based onpeptide mass fingerprinting,52 proteins were identified and classified into seven functional groups:Cell/organism defense and antioxidant,cell signaling/communications proteins,cell structure/motility pro-teins,metabolism proteins,RNA synthesis processing,Protein synthesis and processing,and unclassifiedproteins.The immunoreactivity of GRP78 was detected in GCs in the follicular,and with during GCs Lu-teinizing in the menstrual cycle,the protein expression(brown)increased continually and came to ahead when ovulation happened.Conclusion:This work provides a first step toward the establishment of asystematic ovary protein database and stands as a valuable resource for molecular analyses of normal andpathologic conditions affecting mouse ovaries.
