Protective effect of glycine on hypoxic injury to murine cardiomyocytes
- VernacularTitle:甘氨酸对SD乳鼠心肌细胞缺氧损伤的保护作用
- Author:
Junli ZHOU
;
Yuesheng HUANG
;
Yongming DANG
- Publication Type:Journal Article
- Keywords:
glycine;
cardiomyocyte;
calcium ion;
laser confocal microscopy
- From:Journal of Third Military Medical University
2003;0(23):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the protective effect of glycine (Gly) on hypoxic injury to murine cardiomyocytes and the mechanisms. Methods The survival rate of cardiomyocyte survival was detected by trypan blue exclusion and lactate dehydrogenase (LDH) with an ultraviolet spectrophotometer. Ca 2+ changes in the cardiomyocytes were detected by laser confocal microscopy. Results Glycine markedly improved the survival rate of cardiomyocytes and decreased the release of LDH in cardiomyocytes after hypoxia. The protective effect was in a dose-dependent manner. Glycine could also block calcium overload after hypoxia. Conclusion Glycine has the protective effect on cardiomyocytes through the improvement of survival rate, decrease of LDH release, and blockage of calcium overload after hypoxia.
