Effects of sodium hyaluronate in reformed M-K liquid on prolonging duration of the preserved corneas
- VernacularTitle:改良M-K液中添加透明质酸钠对角膜保存时间影响的研究
- Author:
Juan YANG
;
Min ZHAO
;
Zhiyong XU
;
Hong LI
- Publication Type:Journal Article
- Keywords:
cornea preservation;
sodium hyaluronate
- From:Journal of Third Military Medical University
2003;0(23):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the roles of sodium hyaluronate (SH) in the preservation of corneas. Methods Referred to M-K prescription Preserving liquid MMK was employed as the control. Different concentrations of SH added into the preserving liquid MMK were employed as the experiment groups: group Ⅰ (0.05% SH), group Ⅱ (0.03% SH), and group Ⅲ (0.01%). At 3, 5, 7, and 10 d after preservation in the preserving liquids, the ultrastuctures of the rabbit corneas were observed by scan electron microscopy and light microscopy, the corneas were stained by typan blue alizarin red staining, and the activities of SDH and G-6-P were detected by enzymohistochemistry. Results There was difference in macrostructure of the corneas in each group. Morphological changes in group Ⅰ were not obvious, but those in the control group were remarkable. Trypan blue-alizarin red staining indicated the highest vitality of corneal endothelial cells (CECs) in group Ⅰ, but the lowest in the control group. In addition, the activities of SDH and G-6-P in each group were significantly different. Conclusion The addition of SH in reformed M-K liquid can prolong the duration of the mid-term preserved corneas and the protective effect is in a dose-dependent manner.
