Isolation purification and characterization of NV-PP-1 from Nidus vespae
- VernacularTitle:露峰房抗炎蛋白中多肽成分的分离、纯化及性质研究
- Author:
Lin LI
;
Xuemei LIU
- Publication Type:Journal Article
- Keywords:
Nidus vespae;
peptide;
isolation and purification;
characterization
- From:
Chinese Pharmaceutical Journal
1999;(4):233-
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVES:To isolate and purify a new peptide named as NV-PP-1,from anti-inflammatory protein extracts of Nidus Vespae and to characterize it.METHODS:It was purified by ammonium sulfate precipitation,DEAE-Sephadex A50 ionexchange chromatography,Sephadex G-50 gel filtration and HPLC，respectively.Then it was characterized by HPLC on Protein-PakTM-60 column,IEF-HPCE(isoelectric focusing-high performance capillary electrophoresis),UV spectrum,amino acid analysis,and the N-terminal analysis with DNS-Cl.RESULT:Both HPLC on Protein-PakTM-60 column and IEF-HPCE showed its homogeneity.Its molecular weight was estimated to be 7.079 kD by HPSEC.UV spectrum indicated its maximum absorption at 274nm.Its PI was determined to be 4.08 according to IEF-HPCE.The terminal analysis showed its N-terminal amino acid was blocked.Amino acid analysis of NV-PP-1 indicated that it was rich in Ser,Glu,Gly.CONCLUSION:NV-PP-1 is the first peptide isolated from the anti-inflammatory protein extracts of Nidus vespae.
