Effect of Cyclosporin A and Tetrandrine on rhodamine 123 accumulationin in bovine brain capillary endothelial cells
- VernacularTitle:环胞素A和粉防己碱对牛脑微血管内皮细胞Rh-123摄取的影响
- Author:
Jinfeng BAO
;
Guoqing LIU
;
Qunyuan XU
- Publication Type:Journal Article
- Keywords:
P-glycoprotein;
brain capillary endothelial cell;
blood-br ain barrier;
rhodamine 123;
cyclosporin A;
tetrandrine
- From:
Chinese Pharmacological Bulletin
2003;0(11):-
- CountryChina
- Language:Chinese
-
Abstract:
Aim To study the effect of cyclosporin A and tetrand ri ne on P-glycoprotein (P-gp)of bovine brain capillary endothelial cell. M ethods The fluorescent dye, rhodamine-123 (Rh-123) was used to evaluate t he functional activity of the P-glycoprotein (P-gp) efflux transport system in primary cultured bovine brain capillary endothelial cell (BCEC) monolayer. Results Rhodamine-123 accumulation was increased significantly in monola yer treated with the P-gp modifying agent, cyclosporin A and tetrandrine. Conclusion The observation suggests that this Rh-123 method is sens itive, stable to evaluate the function of P-gp of blood-brain barrier (BBB). R h-123 accumulation is also increased by tetrandrine in dose-dependent manner.
