The effects of methotrexate on vascular smooth muscle cells proliferation, migration and apoptosis
- VernacularTitle:甲氨喋呤对血管平滑肌细胞增殖、迁移和凋亡的影响
- Author:
Lei MENG
;
Qingping TIAN
;
Yanhong GUO
;
Zhuo YU
;
Lin XUE
;
Wei GAO
- Publication Type:Journal Article
- Keywords:
methotrexate;
smooth muscle cell;
proliferation;
migration;
apoptosis
- From:
Chinese Pharmacological Bulletin
2003;0(11):-
- CountryChina
- Language:Chinese
-
Abstract:
Aim To observe the effect of methotrexate (MTX) on proliferation, migration and apoptosis of cultured vascular smooth muscle cells (VSMC). Methods Rabbit thoracaortic VSMC were cultured in vitro.VSMC proliferation was evaluated by cell counting and cell cycle analysis. Monolayer cell scrape was used to observe VSMC migration. Apoptosis was observed with flow cytometry, DNA gel electrophoresis and TUNEL stain. Results MTX (25～100 nmol?L -1) inhibited VSMC proliferation in a dose-dependent manner.25 nmol?L -1 and 50 nmol?L -1 MTX increased the percentage of the S phase cells and decreased the percentage of the G 2/M phase cells (P
