Effects and mechanisms of intraarticular injection of doxycycline on experimental osteaarthritis in rabbits
- VernacularTitle:强力霉素治疗兔实验性骨关节炎的疗效及其作用机制的研究
- Author:
Liang SHANG
;
Liaobin CHEN
;
Xiannian ZHENG
;
Peng WANG
- Publication Type:Journal Article
- Keywords:
osteoarthritis;
doxycycline;
NO;
NOS;
MMP-13;
therapy
- From:
Chinese Journal of Clinical Pharmacology and Therapeutics
2004;0(11):-
- CountryChina
- Language:Chinese
-
Abstract:
AIM: To investigate the effects and mechanisms of intraarticular injection of doxycycline on experimental osreoarthritis in rabbits. METHODS: An animal model of osteoarthritis in knee of rabbits was established by intraarticular injection of papain. 2 mg or 4 mg of doxycycline was injected (intraarticular) once a day in four weeks. The degeneration of articular cartilage, Mankin s marks of the cartilage tissue, the expression of MMP-13, the release of nitricoxide (NO) and the activity of total nitricoxide synthase (NOS) and inducible nitricoxide synthase (iNOS) of the joint fluid were tested subsequently . RESULTS: In the osteoarthritis model group, the Mankin s marks and the expression of MMP-13 observably increased, and spectrophotometric analysis showed the high concentration of the release of NO and high activity of NOS and iNOS in the joint fluid (P
