Effects of low-dose theophylline on differentiation of nave T lymphocyte
- VernacularTitle:小剂量茶碱对原始T淋巴细胞分化的影响
- Author:
Hanxiang NIE
;
Wei GUO
;
Jiong YANG
- Publication Type:Journal Article
- Keywords:
pharmacodynamics;
asthma;
theophyl-line;
Th1 cells;
Th2 cells;
cytokine
- From:
Chinese Journal of Clinical Pharmacology and Therapeutics
2004;0(11):-
- CountryChina
- Language:Chinese
-
Abstract:
AIM: To investigate the effects of low-dose theophylline on differentiation of the naive T lymphocyte. METHODS: Naive T lymphocytes and monocytes were isolated from cord blood in neonate. Theophylline (10 mg?L-1 ) was added in naive T lymphocytes and monocytes which stimulated by PHA (200 mg?L-1 ) for 3 days, and the differentiation of T cells was analyzed by flow cytometry. Cytokines were measured by enzyme linked immune-sorbent assay ( ELISA ). RESULTS: Theophylline significantly promoted differentiation fromTh1 cells to naive T cells ( P
