Inactivation of HIV-1 in whole blood by methylene blue photochemical method
- VernacularTitle:亚甲基蓝光化学法对全血中人免疫缺陷Ⅰ型病毒的灭活研究
- Author:
Yao ZHANG
;
Hongyan XIONG
;
Fei MA
;
Zuoy BAO
- Publication Type:Journal Article
- Keywords:
methylene blue;
photochemical method;
HIV-1
- From:Journal of Third Military Medical University
2003;0(21):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To observe the ability of inactivating human immunodeficiency virus type 1 (HIV-1) in whole blood by visible light combination with methylene blue (MB) at different concentrations. Methods HIV-1 was used as the test virus. The contaminated blood was treated by MB, visible light (640 nm), combined with MB and visible light (640 nm). The test of MT4 cell infection was used to evaluate the virus inactivation efficacy. Results After being treated by MB at the concentrations of 5, 10, and 15 ?mol/L, and then irradiated respectively by visible light (40 000 Lux, 640 nm) for 30, 20, and 10 min, all the added indicated virus at the titer of 10 5.78 TCID 50 of HIV-1 could be inactivated absolutely. Conclusion Methylene blue photochemical method can inactivate HIV-1 in blood effectively.
