Establishment of a novel epithelial cell line from laterally spreading tumor of colon
- VernacularTitle:大肠侧向发育型肿瘤细胞株的建立及其鉴定
- Author:
Zhuosheng LAI
;
Yujing HAN
;
Side LIU
- Publication Type:Journal Article
- Keywords:
laterally spreading tumor, rectocolon;
cell isolation;
cell culture;
cellular histochemistry;
biological characteristic
- From:
Medical Journal of Chinese People's Liberation Army
2001;0(11):-
- CountryChina
- Language:Chinese
-
Abstract:
Objectives In order to further investigate the biological characteristics and the developmental mechanisms of laterally spreading tumor, a cell line from the primary culture of human colon LST tissue was isolated. After 70 passages, we identified the cells by morphologic, genetic and immunohistochemistry analysis. Results (1) The origin of LST cells is epithelial, and the majority of which were multiform epithelial cells. It displayed an approximately similar growth characteristics of tumor cells. The nest corpus doubling time was 36 hours. (2) The number of chromosome is 42-66. Eighty-five percent of chromosomes were triploid, showing abnormal karyotypes. (3) Immunohistochemistry showed that ESA and CK20 were expressed. (4) The ultrastructure of LST was almost the same as that of a tumor cell. Conclusion A novel LST cell line has been established and named as LST-R1. It facilitates further study of the biological characteristics of LST cells and the development of colon cancer.
