Effect of several anti-ulcer drugs on the apoptosis and proliferation of gastric mucosa cells in development of stress ulcer in rat
- VernacularTitle:大鼠应激性溃疡发病过程中不同抗溃疡药物对细胞凋亡和增殖的影响
- Author:
Jing LIU
;
Zhaoshen LI
;
Xinjian WAN
- Publication Type:Journal Article
- Keywords:
stress ulcer;
apoptosis;
proliferation;
omeprazole;
talcid;
misoprostol
- From:
Medical Journal of Chinese People's Liberation Army
2001;0(11):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the effect of antiulcer agents Omeprazole, Misoprostol and Talcid on the gastric mucosa lesion by analyzing the gastric mucosa cell apoptosis and proliferation and CX43 transcriptional expression. Methods Mucosa lesion were evaluated by UI, 32 mice were randomly divided in equal numble into Omprazole (O), Misoprostol (M), Talcid (T), Comparison (C). Apoptosis cells in gastric mucosa were quantitated by terminal deoxynucleatidyl transferase mediared dUTP nick and labelling (TUNEL) techniques, while the expression of PCNA proteins were detected by immunohistochemical staining, CX43 mRNA was detected by In situ hyberdization. at 2h after WRS. Results We found that in comparison with control group, the group of pretreatment with Omeprazole, Misoprostol and Talcid showed a significant reduction in damaged mucosa and epithelial cell apoptosis and increase in the expression of PCNA proteins, and the effect of Omeprazole and Misoprostol was better than Talcid. All three antiulcer agents increased CX43 mRNA expression, the effect of Misoprostol and Talcid was better than Omeprazole. Conclusions Omeprazole, Misoprostol and Talcid can attenuate the multiple gastric mucosa lesion induced by WRS. It may be the ultimate way to prevent and cure stress ulcer by exerting direct cytoprotective effect to improve the cell ability and inhibit stress injury.
